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Secondary antibodies were then used for detection as follows: AlexaFluor488 goat anti-rabbit (1:500, Invitrogen, A11008), AlexaFluor647 goat anti-rabbit (1:500, Invitrogen, A21244)

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As per the guidelines, only qualified specialistssuch as endocrinologists, internal medicine physicians, and cardiologistsare authorised to prescribe GLP-1 drugs