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cAMP T EPAC VV Fluorescence Resonance Energy Transfer (FRET) imaging Islets extracted from Pdx1 Cre-ERT -CAMPER mice, conditionally expressing the T EPAC VV cAMP biosensor in beta cells under the control of the Pdx1 promoter conjugated to a mutant oestrogen receptor sequence (63), were incubated in 1 M 4-hydroxytamoxifen for 24 hours to induce T EPAC VV expression, treated with simvastatin as above, washed, encased in Matrigel and imaged in 6 mM glucose KRBH buffer using a Zeiss LSM-780 inverted confocal laser-scanning microscope in a 20X objective at 37C

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Br Med Bull (2011) 99:3951

Supplementing with glutathione is a marathon, not a sprint

These mutations may lead to the accumulation of abnormal metabolites, which can potentially affect the redox balance and energy production in TC cells, ultimately contributing to tumor progression and potentially serving as a biomarker for TC diagnosis and prognosis

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