Griffin, J
The GAPDH substrate 1,3 bisphosphoglycerate was generated by incubating 13.5 mM 3-phosphoglycerate, 6 mM ATP with 0.012 U/l 3-phosphoglycerate phosphokinase in 100 mM Tris/HCl p H 8.0, 1 mM EDTA, 15 mM MgCl 2 for 20 to 30 min at RT
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2 Other commercially available forms include the naturally occurring methyl-B12, and less commonly adenosyl-B12, and hydroxo-B12, which is used mainly in prescription products
Journal of Alternative and Complementary Medicine
(13, 14, 15) Not only is it difficult to make, but once its made, it is constantly used up because of your exposure to lifes many toxins and stress