Western Blotting Proteins were extracted from chondrocyte (in vitro) and cartilage (in vivo) with cell lysis buffer (Pro-Prep, Intron Biotechnology, Korea) and protein concentration was measured using a BCA kit (Thermo Scientific, USA)
However, supplements like berberine can potentiate blood-sugar-lowering effects, potentially causing hypoglycemia when combined with diabetes medications or GLP-1 agonists
ACKNOWLEDGEMENTS We thank Peggy Kroll-Conner for help generating strains central to this work as well as isolation of the puf-3(q801) deletion, Maureen Barr for sharing her mutagenesis library, and the Million Mutations Project for generating puf-11(gk203683)
This "L" refers to the specific chemical shape or "handedness" of the molecule
doi:10.1111/j.1365-2133.2008.08916.x (Epub 2008 Nov 11)
Fluids 26 , 041902 (2014) A.Z.K