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To determine the expression of translocated Nrf2 and NF-B in the nucleus, and the levels of Nrf2, Keap1, heme oxygenase-1 (HO-1) and p-IB in the cytoplasm, nuclear and cytoplasmic extracts from the lung tissue were prepared with a previously described method (Rajendrasozhan et al., 2010)

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However, very large VLDL particles cannot be secreted if their diameter exceeds that of the sinusoidal endothelial pores, and this limitation may ultimately result in lipid retention and NAFLD [120]

The inclusion of KPV is a key differentiator