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This work was supported by grants from the Deutsche Forschungsgemeinschaft to BG (GR 936/171 and 17-2) and by the Collaborative Research Center SFB1078 (Humboldt Universitt zu Berlin, TP A5 to RH)

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Comparing the sgRNA distribution in the non-treated cells with the library plasmid stock showed that several sgRNAs targeting essential control genes and essential membrane transporters led to a growth reduction (Fig S2B), confirming a high KO efficiency in this cell line and experimental setup

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As always, it is recommended to consult with a healthcare practitioner to determine if this product fits your specific health needs