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Subsequently, the peak fraction corresponding to the target 6-hydroxy-FAD was collected, supplemented with methanol at a final concentration of 5% (v/v) and acetic acid at a final concentration of 1% (v/v), loaded onto a supelclean LC-18 SPE column (Sigma-Aldrich), washed with a solution containing 5% methanol and 1% acetic acid, and water in that order, and then eluted by using 100% methanol