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Mass spectrometry (MS) has long been used for the identification and determination of the molecular structure of organic compounds (Khalikova et al., 2024)

Samples were denatured by boiling at 95 C for 10 minutes, loaded onto a 4% to 12% gradient gel (Thermo Fisher Scientific), and separated by electrophoresis

The reconstitution process is essentially identical, but there are a few practical differences worth noting

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The sequence is derived from a protein found in human gastric juice

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