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The cell suspension was sonicated using mechanical ultrasonicator (4 s intervals of 50 Hz repeated 10 times) followed by 3 cycles of freezing of cell lysate in liquid N 2 and thawing at room temperature

The clinical disease course in the IBD-PSC population is often mild, with ulcerative colitis-PSC patients requiring fewer steroids and colectomies than those with ulcerative colitis alone [211]

Months 36: Continued weight loss at a pace of 0.51.5 lbs per week at full dose

[15] The two main melatonin receptor subtypes, designated MT1 and MT2, are G-protein coupled receptors that mediate the majority of melatonins physiological effects

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