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(2023) 186:274864.e22

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This formulation elicited stronger IgG and secretory IgA responses than mRNA-loaded liposomes and retained functional stability at room temperature for up to 1 month

A clinical study of alopecia areata in Mexico

Quantification of IL-6, IL-8, IL-1 and PGE2 by enzyme-linked immunoassay (ELISA) Supernatants obtained from the qPCR assay were centrifuged at 600 g for 6 min and used to estimate concentration using bovine IL-8 ELISA kits (#3114-1A-6, Mabtech, Nacka, Sweden), IL-6 (#ESS0029, Thermo Fisher Scientific), IL-1 (#ESS0027, Thermo Fisher Scientific) and PGE2 (#514010, Cayman Chemicals, Ann Arbor, MI, USA) according to the manufacturer's instructions