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In brief, 30 ml of the HEK293 cell suspension culture in a 50 ml centrifuge tube (Violamo, #VIO-50BN) was centrifuged sequentially at 1000 g for 10 min and 3000 g for 10 min at 4 C to remove cell bodies and dead cells, respectively, using MX-307 centrifuge and AR510-04 rotor (TOMY, k-factor: 880)

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Amino Acids Contained Both tripeptides use histidine and lysine to bind copper, but they differ in the first amino acid in their sequence

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ACA, given at doses of 25 and 50 mg/kg/day to the animal model, suppressed the IgE level and the infiltration of eosinophils in the lungs