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Immunoblotting For immunoblotting, the cells were lysed with RIPA buffer, and the protein concentrations were determined

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For preparation of reduced controls, 100 M -mercaptoethanol was added and incubated under identical conditions

[DOI] [PMC free article] [PubMed] [Google Scholar] 63.Probst L., Monnerat S., Vogt D.R., Lengsfeld S., Burkard T., Meienberg A., Bathelt C., Christ-Crain M., Winzeler B