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A 2026 cardioprotection study using a permanent LAD coronary artery ligation model in Sprague-Dawley rats evaluated GHRP-6 at 0.4 mg/kg (established minimum effective dose for inotropic enhancement)

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The body makes its own collagen, but around age 35, this production begins to decline and signs of aging start to show

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After overnight incubation at 4C, wells were washed thrice with PBS and blocked with 1% fatty acid free BSA (Sigma) in PBS for 1h at room temperature (RT)

Kheder et al., 2017