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After binding your GST-tagged protein to glutathione beads, you can use this as a bait to search for interacting proteins

The two Asp's act as the main catalytic agents, and together with a water molecule cleave the protein chain bound in the tunnel

TBARS levels increased twofold (0.36 mmol/mg protein) in diabetics compared to healthy controls (0.15 mmol/mg protein)

N' -Acetyl-L-Cysteine (NAC) can exist in the reduced form, containing the sulfhydryl (-SH) group, and it can exist in its oxidized disulfide form N,N' -Diacetyl-L-Cystine (Di-NAC)

We can't stress this enough: the reconstitution step is an active and critical part of your experimental procedure, not just a prep-work task to rush through

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