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However, using a proteomic approach on a subset of placental samples, numerous mitochondrial proteins were found to be down-regulated in PE3 placentas compared with controls, including: subunits of OXPHOS protein complexes (NADH:Ubiquinone Oxidoreductase Subunit V3 [NDUFV3], Ubiquinol-Cytochrome C Reductase Core Protein 1 [UQCRC1], and UQCR Rieske Iron-Sulfur Polypeptide 1[UQCRFS1]), and proteins that affect mitochondrial dynamics (optic atrophy type 1 [OPA1]), mitochondrial import (TOMM7), and mitochondrial translation (mitochondrial ribosomal proteins, MRPS36, MRPL44, MRPS36, MRPL22, MRPL1), among others (Fig 1H)
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GAPDH was used for normalization, and experiments were repeated a minimum of three times
For extended storage periods, we recommend freezer conditions to maintain long-term stability
It is best used as part of a comprehensive recovery plan
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