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Kodarahmian M, Amidi F, Moini A, Kashani L, Nashtaei MS, Pazhohan A, et al

Protein expression For whole cell lysates, cultured cells were rinsed with ice-cold PBS and lysed in RIPA buffer (150 mM NaCl, 50 mM Tris-HCl pH 8.0, 5 mM EDTA, 1% NP40, 0.5% sodium deoxycholate, 0.1% SDS supplemented with PhosSTOP and cOmplete protease inhibitor cocktail

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Berryman and colleagues revised IGF-1 actions on obesity, and concluded that this molecule possesses direct effects on muscle glucose uptake [160]

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