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Histology A 10-mm long piece of the spinal column (the L2L3 vertebral body) and the surrounding muscle were collected from each sacrificed animal and fixed in 4% formaldehyde in phosphate buffer (pH 7.4)

Details regarding sex and age and cohort size of the recruited participants can be found in the population characteristics section of this manuscript

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The Broad Institute, a genomic research center run by MIT and Harvard, maintains a database called the Connectivity Map that tracks how compounds shift gene expression across thousands of human cell lines

Special Populations While comprehensive data in special populations is limited, consider the following: Geriatric patients: Start at lower doses (200 mcg daily) given potentially altered pharmacokinetics