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Quantification reactions were performed in triplicate for each sample using the Ct method with hypoxanthine-guanine phosphoribosyltransferase ( hprt ) serving as the reference housekeeping gene

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These effects promote angiogenesis, fibroblast activity, and neuromuscular stabilization, particularly in poorly vascularized tissues such as tendons and myotendinous junctions

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By week 3 of rest, improvement plateaus