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This shift can contribute significantly to body fat reduction

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After washing five times with lysis buffer, the complexes were mixed with lysis buffer containing 290 mM imidazole, and the bound proteins were eluted by boiling in 5 sodium dodecyl sulfate (SDS) loading buffer for 5 min

Chemico-Biol Int 322:108968 Li Y, Glotfelty EJ, Karlsson T, Fortuno LV, Harvey BK, Greig NH (2021) The metabolite GLP-1 (936) is neuroprotective and anti-inflammatory in cellular models of neurodegeneration