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After being rinsed with PBS, the sections were treated with 3% H 2 O 2 for 15 min to quench endogenous peroxidase activity, permeabilized and blocked with 0.3% Triton X-100 in PBS containing 5% BSA for 1.5 h at room temperature

The plates were stored in a 5% CO 2 incubator at 37C overnight

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