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The mixture of 10 L RNA and 2 L (150 ng/L) of random hexamer (NNNNNN) was used in the denaturation step at 70 C for 10 min following by the generation of cDNA at 42 C for 90 min using 8 U of AMV Reverse Transcriptase (Promega Corporation, USA) and 200 M dNTPs

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