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The slides were then analyzed with a confocal microscope (Leica SP8), utilizing 488 nm (Alexa 488) and 405 nm (DAPI) lasers under a 63 oil immersion objective

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Over the same period, the number of sleep apnea diagnoses per 1,000 patients rose to 11 from 8 and the number of cardiovascular disease diagnoses increased to 15 from 13