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If you want a more detailed look at this specific question, our antacids-with-semaglutide article covers the basics in more depth
Regarding the refinement for Chc-Apl2.1 we have included secondary structure restraints, NCS torsion restrains, TLS refinement (at the beginning of the refinement one per chain and after the initial cycles the different TLS groups were calculated using phenix), grouped Bfactor refinement using PHENIX (1.21_5207) 58,59
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This dual mechanism is why tirzepatide doses start at 2.5 mg while semaglutide starts at 0.25 mg
A nonpaired t test was used to compare between groups
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