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After reaching 70% confluence, fibroblasts were cultured for 24 h in 1% FBS DMEM and then incubated with TGF1 10 ng/ml (Peprotec, Canada), GHK 10 nM (Sigma, Canada) or growth media control for a further 48 h

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The gap between correct dosing and dosing errors comes down to three calculations most guides gloss over: determining final concentration after reconstitution, converting that concentration to injection volume, and accounting for vial overfill that changes the actual amount of peptide present

Valentino, T

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4% agarose supplied as 50% slurry