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The qRT-PCR primer sets for cDNA amplification were as follows: TNF- (forward: 5-TCCAGGCGGTGCCTATGT-3/reverse: 5-CACCCCGAAGTTCAGTAGACAGA-3), IL-6 (forward: 5-CTGCAAGAGACTTCCATCCAGTT-3/reverse: 5-AAGTAGGG AAGGCCGTGGTT-3), IFN- (forward: 5-TCAAGTGGCATAGATGTGGAAGAA-3/reverse: 5-TGGCTCTGCAGGATTTTCATG-3), CXCL2 (forward: 5-CTCAGTGC TGCACTGGTCCTG-3/reverse: 5-CTGGGGGCGTCACACTCAAG C-3), and GAPDH (forward: 5-CCTCGTCCCGTAGACAAAATG-3/reverse: 5-TCTCCACTTT GCCACTGCAA-3)

The bar graphs represent the mean SD number of primordial, primary, preantral, antral, graafian, and atretic follicles in ovarian tissues from control, GSH, Mel, CARB, GSH+CARB, and Mel+CARB groups

doi: 10.1016/j.ijbiomac.2023.127613

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By doing so, they can enhance natural growth hormone levels, which can aid in muscle development, improve energy levels, support fat loss, and contribute to healthier skin and bones

(60 capsules)