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NDI1 expression did not have any major effects on gene expression in brown adipose fat (BAT) and iWAT (Extended Data Fig

Unbound proteins were washed out extensively with the following buffers stepwise: 1% Triton X-100, 500 mM NaCI, 1 mM NEM, 1 M Urea in 1x TBS, 1% Triton X-100, 1 mM NEM in 1x TBS, and 1% Triton X-100 in 1x TBS

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Similar findings were reported in analyses of tirzepatide, where about three-quarters of the weight loss was fat mass and one-quarter was lean mass, proportions that closely resemble those observed with diet-induced weight loss [14, 23]