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Rev Bras Hematol Hemoter

400 HFD is the standard method to induce obesity in animal models and results from the overconsumption of fat, which directly increases caloric intake

Statistical analysis Data (means SD) were analyzed using SPSS software (SPSS Inc., Chicago, IL, USA)

Additionally, Gammaproteobacteria, recognized as opportunistic pathogens, increases susceptibility to intestinal infections and exacerbate inflammation-driven metabolic dysfunction, impairing thyroid hormone utilization (Yao et al., 2020)