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1.2 kg [SD: 3.2], P = 0.005)

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coli BL21 cells were transformed with the plasmid containing Nb35 target gene and were grown in TB medium with 100 g/mL ampicillin, 1 M MgCl 2 , 2% (w/v) glucose at 37 C, 180 rpm for 3 h

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