A peptide calculator can help simplify this process
Double-click a preset to edit it Double-click a preset to edit it Double-click a preset to edit it Ultra-fine, best for small doses recommended for 5-Amino-1MQ Concentration 10.00 mg/mL Draw volume 0.50 mL = 5 mg 10.00 mg/mL Units to draw (0.3 mL syringe) 25 units 2 shots Your 0.3 mL syringe only holds 30 units
The following primary antibodies were listed: -SMA (1:2000, Abcam Cat# ab7817), LRP1 (1:2000, Abcam Cat# ab92544), LRP1 (1:2000, Sigma-Aldrich Cat# L2295), COL1a1(1:2000, Cell Signaling Technology Cat# 72026), ECM1 (1:2000, Sigma-Aldrich Cat# HPA027241), Flag M2 (1:2000, Sigma-Aldrich Cat# F3165), HA-Tag (1:2000, Cell Signaling Technology Cat# 3724), Fibronectin (FN) (1:2000, Cell Signaling Technology Cat# 26836), GST(1:2000, Cell Signaling Technology Cat# 2622), Strep-tag II(1:2000, Abcam Cat# ab184224), AKT(1:2000, Cell Signaling Technology Cat# 4691), p-AKT(1:2000, Cell Signaling Technology Cat# 4060), mTOR(1:2000, Cell Signaling Technology Cat# 2983), p-mTOR(1:2000, Cell Signaling Technology Cat# 5536), FoxO1(1:2000, Cell Signaling Technology Cat# 2880), -Actin (1:5000, Cell Signaling Technology Cat# 4967) and -Tubulin(1:5000, Cell Signaling Technology Cat# 2144)
Based on analysis of protein as determined by an independent laboratory using the SDS PAGE Gel method
DAPI fluorescent dye (C1005, beyotime, Shanghai, China) was used to stain the cell nuclei
first found that I/R-induced kidney ferroptosis was mediated by augmenter of liver regeneration (ALR) via the GSH/GPX4 axis [43]
