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[DOI] [PubMed] [Google Scholar] 49.Aronne L.J., Sattar N., Horn D.B., et al

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The protein was concentrated using Amicon Ultra-4 Centrifugal Filter Units with a 3 kDa membrane cutoff (Merck) and then acidified to a pH of 2.2

The number of amino acids is not a fixed limit but scientists have an idea that molecules with over, say, 50 amino acids are classified as proteins