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The sorted cells were washed twice with 50 mL phosphate-buffered saline to remove the FreSHtracer and then further cultivated in culture medium for 24 h prior to in vitro or in vivo experiments, as described below

doi: 10.3389/fimmu.2017.01005 153 LiuZ.WangM.ZhangC.ZhouS.JiG

This upregulation persists for up to three days after treatment, with the combination of BPC-157 and growth hormone synergistically enhancing cell proliferation and PCNA expression

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Whether combining these specific compounds produces additive or synergistic effects has not been formally studied, but the non-overlapping mechanisms provide a theoretical rationale