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Biotin (1 mM in PBS, sterile filtered) was added to a final concentration of 50 M at the indicated timepoint, and cells were fixed and stained 24 h after seeding

doi: 10.1016/j.joca.2023.01.573 167 GuoPGaoXNelsonALHuardMLuAHambrightWSet al

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It typically lessens significantly over time

Calcium intake is not associated with increased coronary artery calcification: the Framingham Study

Copper delivery through the skin has many potential applications and thus is widely studied, including the evaluation of the penetration ability of copper peptides 30 , biocompatibility of copper containing IUD 1 , the enhancement of skin absorption through copper-containing nanoparticles 10 and microneedle-mediated delivery 5