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The NGs were treated 20 min at 37 C with 0.10.5 mg ml 1 collagenase Ia (Sigma), 0.40.6 mg ml 1 dispase II (Roche, Basel, Swiss), 15 g ml 1 DNase II type IV (Sigma), and 0.75 mg ml 1 bovine serum albumin (Sigma) in HKRB supplemented with 5.6 glucose