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Moreover, the MDSCs proportion of tumors following the ZFPG + US treatment (11.89%) was significantly lower than that of the ZFPG (27.36%) and ZFP + US (24.56%) treatments, indicating that the ZFPG + US treatment was the most valid in alleviating the immunosuppressive TME (Fig

NAD+/NADH ratio assays, SIRT1 activity assays, and mitochondrial respiration measurements (such as oxygen consumption rate via extracellular flux analysis) are standard methods for studying this combination's proposed effects in cell-based models, typically comparing single-compound and combined-compound treatment conditions against untreated controls

Many patients remain on treatment for a year or longer

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Experimental evidence suggested cytotoxic T cells suppress neural stem cell proliferation via interferon- (IFN-) secretion (Li et al., 2023)