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Furthermore, there was a significant 8-fold increase in the growth of BCG inside granulomas from T2DM subjects compared to the healthy subjects ( Figure 2B )

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Alcohol Alcohol 2010;45(5):449-455

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The post-opening discard period exists because: Sterility can no longer be fully guaranteed after first puncture Preservatives slow microbial growth but do not eliminate contamination risk Risk of contamination increases with repeated vial access For this reason, healthcare and laboratory settings typically follow a fixed discard timeframe (commonly 28 days), unless otherwise specified by the manufacturer