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Make sure to hydrate more frequently during high blood sugar episodes

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coli BL21(DE3) cells carrying the plasmid pET-BirA (protein sequence: His 6 -SSGLVPRGSH-BirA) 82 (a kind gift from Professor Ying Gao at the authors institute) were induced with 0.1 mM IPTG for 15 h at 18 C

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