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Following incubation (total of 72 h), the plates were frozen at 80C for at least 1 h

The excitation filter for CFP was 430/20 nm and emission filters for CFP and YFP were 485/20 nm and 535 nm/25 nm, respectively in microplate reader

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Dilokthornsakul W, Dhippayom T, Dilokthornsakul P

Biochemistry 46 , 14321447 (2007)