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Quantification of GSH using LCMS Cells were incubated with 100 M of N -methyl maleimide (NMM) in PBS for 15 min at room temperature to derivatize all the small-molecule thiols

Each 0.25mL injection delivers exactly 5mg

ABB Acid-base balance APV Artificial pulmonary ventilation CRP C-reactive protein CSF Cerebrospinal fluid CT Computer tomography ENT Ear-nose-throat GPx Glutathione peroxidase GR Glutathione reductase GCS Glasgow Coma Scale NADPH Nicotinamide adenine dinucleotide phosphate, reduced form PEG Percutaneous endoscopic gastrostomy ROS Reactive oxygen species SOD Superoxide dismutase

Results Between 10 and 20 mg/kg dose, median AUCs of acetaminophen, glucuronide, sulfate, and cysteine increased significantly resulting in unchanged ratios of AUC of metabolite to acetaminophen

No safe or effective human dose has been established [10]

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