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2020;22(1):19m02504
Blots were blocked in either 5% (w/v) nonfat milk (Lab Scientific, M-0841) or 1% (w/v) BSA in 1 Tris-buffered saline with Tween (TBST) (Teknova, T1688) for 3060 min at room temperature, incubated with primary antibodies in TBST for 1 h at room temperature, washed three times with TBST for 5 min each, incubated with fluorophore-conjugated secondary antibodies for 30 min at room temperature, washed three times with TBST and imaged on an Odyssey CLx imager (LI-COR) or ChemiDoc XRS+ imager with Clarity western enhanced chemiluminescence blotting substrates (Bio-Rad)
Eggs Egg, large: One egg provides 6 grams of protein
NNMT is increased in a broad range of cancers, such as papillary thyroid cancer, renal clear cell carcinoma, glioblastoma tumors, bladder cancer, colorectal cancers, gastric cancers, and oral squamous cell carcinoma
By inhibition of histone deacetylase, it promotes more transcriptionally active chromatin structures, that is it exerts an epigenetic effect
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