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This protocol has since been adapted with direct differentiation of iPSC to brain endothelial cells using a chemically defined medium (Qian et al., 2017)

2015 Apr 21;5(2):545-89

Zhang Z, Wu Y, Yuan S, Zhang P, Zhang J, Li H, et al

Emerging applications of nanomedicine for the diagnosis and treatment of cardiovascular diseases

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The rationale is that TRT stabilizes systemic testosterone while BPC 157 supports tissue healing and vascular health, potentially mitigating training-related injuries and TRT-linked blood pressure changes