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Manser, A
An aliquot from the wells containing the most active LacAnc100 and LaccID clones was inoculated in 3 ml of YPD (10 g l 1 yeast extract (Gibco, 212750), 20 g l 1 peptone (Gibco, 211677), 20 g l 1 d-glucose (Thermo Scientific, 410955000) and 25 g ml 1 chloramphenicol (Merck, C0378)) and incubated at 30 C and 225 rpm for 24 h
Mean FPG and glycated hemoglobin (HbA 1c ) decreased significantly with all liraglutide doses versus placebo at year 1 and with liraglutide 2.4/3.0 mg versus orlistat at year 2 (Supplementary Figure 3)
Sunny NE, Bril F, Cusi K
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