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Cells were seeded at a density of 5000 cells per well in 200 l fresh culture medium (DMEM supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin solution) into 96-well flat-bottomed microtitre plates (Costar, Corning, USA) and incubated at 37 C in humidified 5% CO 2 using AutoFlow NU-5510 Direct Heat CO 2 Incubator (NuAire, USA) for 24 hours before treatment

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The researchers noted that patients who were "responders" required significantly higher doses than standard deficiency protocols to maintain their clinical improvements, suggesting a high cellular demand for the nutrient in the face of chronic oxidative stress

[DOI] [PubMed] [Google Scholar] 164.Gustafson CT, Boakye-Agyeman F, Brinkman CL, et al

doi: 10.3389/fnins.2023.1259473 291 HadannyAZilberman-ItskovichSCatalognaMElman-ShinaKLangEFinciSet al

F., & Pereira, S