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Enzyme-linked immunosorbent assay (ELISA) Concentrations of IL-33 in cell surpernatant and Peritoneal fluid were determined by commercially ELISA systems using the protocol recommended by the manufacturer (biolegend,CA,USA)

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Briefly, these studies revealed that DSePA treatment (at concentrations which are non-toxic for normal cells) inhibited the growth of lung cancer cells by causing reductive stress (marked by the increase in the levels of GSH and GPx) followed by mitochondrial and endoplasmic reticulum-dependent apoptosis [92]